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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways

Fig. 1

The viability of NCI-H1650 cells and NCI-H446 cells and their sublines adapted to hyperthermia treatment. a NCI-H1650 and NCI-H446 cells are cultured under 37, 42, and 47 °C. Apoptosis rate is measured using a Tunnel staining assay. The brown stained cells are apoptocic cells, and semi-quantitative analysis of apoptosis rates are measured. (* p < 0.05 37 °C vs 42 °C heat treatment group, ** p < 0.05 47 °C vs 42 °C heat treatment group) (b) 2 sublines were established following incubation at 42 and 47 °C. The 24, 48, and 72 h viability was evaluated by MTT assay. Parental cells: cells cultured under 37 °C; NCI-H1650-a and NCI-H446-a subline: cells adapted to 42 °C heat treatment; NCI-H1650-b and NCI-H446-b subline: cells adapted to 47 °C heat treatment (* p < 0.05 NCI-H1650-a or NCI-H446-a subline vs parental cells, ** p < 0.05 NCI-H1650-a subline vs NCI-H1650-b subline, NCI-H446-a subline vs NCI-H446-b subline) (c) Growth curve of parental cells and the cells subline a and b are drawn. Data are the representative results of three independent experiments (* p < 0.05 from day 2-7 parental cells vs cells subline-a, ** p < 0.05 from day 2-7 cells subline-a vs cells subline-b). d HIF-1a expression of parental cells, the cell subline a and b are detected by Western-blot and semi-quantitative analysis. (* p < 0.05 parental cells vs cells subline-a, ** p < 0.05 cells subline-a vs cells subline- b)

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