Fig. 4
From: Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways
The effect of wortmannin and PD98095 on HIF-1a expression and angiogenesis potential in NSCLC and SCLC subcutaneous tumors following heat treatment. NCI-H1650-b and NCI-H446-b sublines are subcutaneously injected into nude mice to form transplantation tumors. a Analysis of immunohistochemistry indicates HIF-1a expression in NSCLC and SCLC subcutaneous tumor before and after treatment with wortmannin or PD98095. b According to the results of semi-quantitative analysis, HIF-1a expression in NSCLCs following heat treatment is inhibited after treatment with wortmannin or PD98095. HIF-1a expression in SCLCs is inhibited by wortmannin but does not change significantly with PD98095 treatment (HIF-1a in control group vs wortmannin group: NCI-H1650-b * p < 0.05, NCI-H446-b ** p < 0.05, HIF-1a in control group vs PD98095 group: NCI-H1650-b ※ p < 0.05, NCI-H446-b ※※ p > 0.05) (c) Analysis of immunohistochemistry indicates changes in CD34 expression in NSCLC and SCLC subcutaneous tumors before and after treatment with wortmannin or PD98095. d The results of semi-quantitative analysis show that CD34 expression in NSCLCs following heat treatment is inhibited after treatment with wortmannin or PD98095. CD34 expression in SCLCs is inhibited by wortmannin, but changes little after treatment with PD98095 (CD34 in control group vs wortmannin group: NCI-H1650-b * p < 0.05, NCI-H446-b ** p < 0.05, CD34 in control group vs PD98095 group: NCI-H1650-b ※ p < 0.05, NCI-H446-b ※※ p > 0.05)