Skip to main content
Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways

Fig. 7

HIF-1a expression in SCLCs following heat treatment was regulated through the AKT but not the ERK signaling pathway. The method used was described in Fig. 6; NCI-H446-b subline cells are harvested and western blot analysis for protein expression is applied. a There were no obvious changes in AKT and ERK total protein expression levels ( p > 0.05 between different groups). p-AKT was gradually induced with increasing heregulin concentrations (* p < 0.05 between different groups), but p-ERK expression showed no significant change (# p > 0.05 between different groups). b HIF-1a expression was upregulated after treated by heregulin ( p < 0.05 heregulin group vs control group), but was inhibited after co-treatment with wortmannin, but not PD98059 (* p < 0.05 heregulin group vs heregulin + wortmannin group, # p > 0.05 heregulin group vs heregulin + PD98059 group). Total AKT and ERK protein levels also showed no significant changes between all treatment groups ( p > 0.05 heregulin group vs heregulin + wortmannin group or heregulin + PD98059 group). p-AKT expression was upregulated when treated by heregulin ( p < 0.05 heregulin group vs control group) and inhibited after co-treatment with wortmannin ( p < 0.05 heregulin group vs heregulin + wortmannin group). However, p-ERK expression in SCLCs was unaffected, regardless of heregulin or PD98059 treatment ( p > 0.05 heregulin group vs control group, p > 0.05 heregulin group vs heregulin + PD98059 group)

Back to article page