Fig. 2

TGFβ down-regulates the expression of IL-1R1 in pancreatic stellate cells. a PSCs cultured in the presence of TGFβ, IL-1α or PDGF were analysed by qPCR and IL-1R1 gene expression normalized to unstimulated PSCs. b Protein expression was measured 3 days after exposure to TGFβ and analysed by western blotting. IL-1R1 was quantified by measuring the band intensity (n = 4). c IL-1R1 gene expression was analysed in PSCs incubated in the presence of TGFβ (2 ng/ml) and the TGFβ signaling inhibitor, A-83-01 (5 μM). d PSC expression of SMAD2 and SMAD3 mRNA was assessed by qPCR. e PSCs were stimulated for 3 days with TGFβ (2 ng/ml) in the presence or absence of the SMAD3 inhibitor, SIS3 (5 μM). The gene expression of IL-1R1 was quantified by qPCR and (f) protein expression visualized by western blotting. Error bars represent S.E.M.; *p < 0.05, ** p < 0.005, ***p < 0.001