Fig. 5
TGFβ inhibitors reduce the inhibitory effects of cancer cells on PSCs expression of IL-1R1. a-c The pancreatic cancer cell lines; (a) PC013, (b) BxPC-3 and (c) CAPAN2 were co cultured with PSCs in the presence of IL-1 receptor antagonist (IL-1RA) (10 µg/ml). d-f PSCs were cultured for 3 days in serum free conditioned medium (CM) from (c) PC013, (e) BxPC-3 and (f) CAPAN2 cell lines and TGFβ signaling inhibited by A-83-01 (5 μM) and SIS3 (5 μM). The IL-1R1 gene expression was analysed in PSCs by qPCR and the result shown as relative expression normalized to GAPDH or unstimulated controls. Error bars represent S.E.M.; *p < 0.05, **p < 0.005, ***p < 0.001