Fig. 8
Inhibition of SMAD7 expression in PSCs reduces tumor stroma interactions and decrease cancer cell migration. a-b PSCs were transfected with non-targeting siRNA (control) or SMAD7 siRNA (100nM) for 3 days, followed by 2 days incubation in serum free medium and another 3 days of culture in the presence or absence of IL-1α (1 ng/ml) or (c-d) 12 h of indirectly co-culture with 5×105 BxPC-3 cells and additionally 60 h of culture without any supplements. The supernatants were harvested 5 days post siRNA transfection and transferred to BxPC-3 scratch wound assays. Wound recovery was determined after 24 h and the data was normalized to controls. Error bars represent S.E.M.; ***p < 0.001