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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Heregulin-1ß and HER3 in hepatocellular carcinoma: status and regulation by insulin

Fig. 4

Effects of insulin on heregulin-1ß stimulation of cell migration in HCC cell lines. a Hep3B and Huh7 cells seeded on Transwell® inserts were treated for 24 h with 10−8 M insulin and/or 50 ng/ml heregulin-1ß (HRG-1ß). Migrated cells were enumerated by microscopy following nucleic acid staining with DAPI. b Huh7 cells were submitted to a wound-healing assay by scraping cell layer with a p200 pipet tip and were treated for 48 h with 10−8 M insulin and/or 50 ng/ml heregulin-1ß. The wound closure (% of H0) was measured

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