Brain stereotactic biopsy flow cytometry for central nervous system lymphoma characterization: advantages and pitfalls

Table 2 Flow cytometry analysis of the lymphoid T reactive population from 16 PCNSL SB and corresponding peripheral blood samples

	McAb	% and CD4/CD8 ratio of reactive lymphocytes in SB and PB samples
Case n°		1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16
Brain Streotactic Biopsy	CD3	84	86	91	85	89	98	98	98	95	94	95	98	85	97	87	85
	CD5	75	79	91	75	89	95	97	98	83	96	95	94	85	95	78	85
	CD56	60	2	7	4	4	1	1	5	3	6	1	1	2	40	1	1
	CD3/CD4	31	41	45	26	39	6	18	35	25	59	69	18	60	14	34	32
	CD3/CD8	87	57	50	59	47	72	60	42	74	28	16	71	33	56	53	45
	CD3/CD56	57	4	6	2	6	6	17	5	2	7	1	6	5	42	9	4
	T4/T8 ratio	0.35	0.72	0.9	0.44	0.83	0.08	0.3	0.83	0.34	2.1	4.3	0.25	1.8	0.25	0.64	0.71
Peripheral blood samples	CD19	nd	nd	nd	12	16	40	16	59	nd	29	19	15	nd	nd	11	9
	CD3	nd	nd	nd	64	63	48	55	16	nd	48	54	72	nd	nd	69	89
	CD56	nd	nd	nd	7	21	15	20	17	nd	23	25	20	nd	nd	20	11
	CD3/CD4	nd	nd	nd	73	59	50	79	66	nd	37	51	58	nd	nd	53	57
	CD3/CD8	nd	nd	nd	23	36	46	19	32	nd	62	47	38	nd	nd	43	38
	T4/T8	nd	nd	nd	3.17	1.6	1.08	4.15	2	nd	0.59	1.08	1.5	nd	nd	1.23	1.5

ISSN: 1756-9966