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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Growth differentiation factor 15 contributes to cancer-associated fibroblasts-mediated chemo-protection of AML cells

Fig. 4

GDF15 contributed to the CAF-mediated chemo-protection of the AML cells. (a-c) Western blot, ELISA and Quantitative RT-PCR analyses represented GDF15 expression in the MSC and the CAF cells. d The bar plots showed the relative viability of the THP-1 cells cultured in medium alone or co-cultured with CAFs added with or without an anti-GDF15 antibody under the treatment of Ara-c (10uM) for 48 h. e Quantitative RT-PCR showing GDF15 expression in the CAFs after nucleofection of a GDF15-specific siRNA or a non-targeting control siRNA for 48 h. f The number of viable THP-1 cells cultured in medium alone or direct co-cultured with the layer of CAFs after nucleofection of GDF15-siRNA or not under the treatment of Ara-C (10uM) for 48 h. The data are the result of three independent experiments and are presented as means ± SD,* p < 0.05, ** p < 0.01, *** p < 0.001

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