Fig. 1
Effects of PIM-1 on proliferation, chemosensitivity and the cell cycle. a Knockdown of PIM-1 expression was performed by transfecting PDAC cells with PIM-1 siRNA, and proliferation was detected by CCK8 assay. b PDAC cells transfected with PIM-1 siRNA or control oligos were incubated with different concentrations of gemcitabine for 48 h. Cell viability was evaluated by CCK8 assay, and the inhibition rate at each concentration was calculated. c Transfected cells were treated with different concentrations of erlotinib; the inhibition rate at each concentration was calculated. d MiaPaCa-2 cells transfected with PIM-1 siRNA or control oligos were cultured for 48 h and then collected for cell cycle analysis. The results for the population of cells in each phase of the cell cycle were evaluated by flow cytometry. The data are displayed as the mean ± SD (*, P < 0.05)