Fig. 4

PIM-1 and the EGFR signalling pathway formed a feedback loop. a PDAC cells were transfected with PIM-1 siRNA and control oligos for 48 h, and protein levels of PIM-1, EGFR and p^Tyr1068-EGFR were detected using western blots. b PDAC cells were treated with different concentrations of erlotinib to block the EGFR pathway, and protein levels of PIM-1, EGFR and p^Tyr1068-EGFR were detected using western blots. c Knockdown of EGFR expression using siRNA. Western blots were used to detect protein levels of PIM-1, EGFR and p^Tyr1068-EGFR