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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Interplay between intergrin-linked kinase and ribonuclease inhibitor affects growth and metastasis of bladder cancer through signaling ILK pathways

Fig. 1

ILK and RI expression is determined by Western blot and Immunofluorescence after transfection for 48 h. a Immunofluorescent observation of ILK and RI was detected respectively. EJ-ILK cells demonstrated remarkably stronger immunofluorescent signal in cytoplasm whereas EJ-RI cells demonstrated significantly stronger immunofluorescent signal in nucleus, compared with the other control groups respectively (magnification × 200). b & c ILK and RI protein expression level was evaluated by Western blot. Proteins were separated by SDS-PAGE and then binded to specified monoclonal ILK antibodies or polyclonal RI antibodies. β-actin was used as control. The levels of ILK proteins were remarkably increased by transfected with pCMV-3 × FLAG-ILK and the levels of RI proteins were significantly increased by transfected with lentivirus containing RNH1 (LV-RNH1), compared with the control groups respectively

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