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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Interplay between intergrin-linked kinase and ribonuclease inhibitor affects growth and metastasis of bladder cancer through signaling ILK pathways

Fig. 2

RI interacts with ILK in vivo and in vitro. The interaction of RI with ILK was detected as described in “Materials and methods” with GST pulldown and co-immunoprecipitation (Co-IP). a & b The interaction of RI with ILK was determined by GST pull down. The bacteria were induced with 0.1 mmol/L IPTG in different time, and then the fusion protein was obtained after ultrasonic decomposition and washed repeatedly. Western blot showed that ILK protein from EJ cells (a) or 293 cells (b) transfected with pCMV-3 × flag-ILK and pCMV-3 × flag could be combined with GST-RI and be specifically pulled down. c & d RI was co-immunoprecipitated with ILK in EJ cells (c) or 293 cells (d). The interaction of RI with ILK was detected as described in Methods, the co-immunoprecipitation of RI and ILK suggested that ILK could specifically bind to RI in the cells

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