Fig. 4
Cell morphology, cell cycle and cell proliferation are observed. a The observation of living cell was executed (magnification × 200). The EJ-ILK group’s cells showed mesenchymal a characteristic and more division phases compared with some cells in the other control groups. On the contrary, EJ-RI cells displayed an epithelial morphology and less division phases compared with some cells in the other control groups. b Flow cytometry was conducted with propidium iodide staining. Experiments were repeated three times, samples were analyzed by Student’s T-test and statistical significance with *P < 0.05. c Flow cytometry analysis of cell cycle distribution demonstrated that the percentages of the EJ-RI cells in S phase were significantly increased, whereas the percentages of EJ-RI cells in G1 phase were decreased in comparison to EJ cells or EJ-LV5 cells. In addition, a slight increase in G1 phase and a decrease in G2/M phase were detected in EJ-ILK cells. d 1.5 × 103 cells were added into a 96 well plate, the CCK8 assay was performed and recorded the A450 with a plate reader every 24 h. Each point represents the mean of twelve independent samples and experiments were repeated 3 times. EJ-RI cells showed inhibited proliferation abilities, whereas increased proliferation abilities were detected in EJ-ILK cells, compared with control group cells respectively, *P < 0.05