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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Interplay between intergrin-linked kinase and ribonuclease inhibitor affects growth and metastasis of bladder cancer through signaling ILK pathways

Fig. 7

Up-regulating RI and ILK regulates the expressions of ILK signaling pathway molecules and EMT-related proteins in vivo. a Immunofluorescent sections were stained in tumor xenograft tissue with rabbit anti-human p-Akt (S473), p-GSK3β (S9) p-PI3K, p-PTEN, p-mTOR, β-catenin, E-cadherin, MMP2, MMP9 and Vimentin respectively, then were incubated with Alexa Fluor 594 Goat Anti-Rabbit IgG or Alexa Fluor 488 Goat Anti-Rabbit IgG secondary antibody. EJ-ILK cell groups resulted in significantly higher p-Akt, p-GSK3β, p-PI3K, p-PTEN, p-mTOR, β-catenin, MMP2, MMP9 and Vimentin as well as lower E-cadherin expressions in tumor tissue, compared with EJ and EJ-FLAG cells groups. In contrast, weaker p-Akt, p-GSK3β, p-PI3K, p-PTEN, p-mTOR, β-catenin, MMP2, MMP9 and Vimentin expression were detected in tumor tissue of the EJ-RI cell groups along with stronger E-cadherin expressions, which was in accordance with experiments in vitro (magnification × 200). b Immunohistochemical sections stained with mouse anti-human ILK, rabbit anti-human RI, p-Akt(S473), p-GSK3β(S9), p-mTOR, β-catenin, E-cadherin, MMP2, MMP9 and Vimentin respectively, the nuclei were counterstained by hematoxylin (magnification 400×). Representative images demonstrated that the EJ-RI cell groups had a weaker brown immunostain for ILK, p-Akt, p-GSK3β, p-mTOR, β-catenin, MMP2, MMP9 and Vimentin as well as a stronger positive signal for RI and E-cadherin in cytoplasm compared with the control group cells respectively. EJ-ILK cells group revealed much lower RI and E-cadherin expressions as well as stronger positive signal of ILK, p-Akt, p-GSK3β, p-mTOR, β-catenin, MMP2, MMP9 and Vimentin expressions in tumor tissue (magnification × 400)

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