Fig. 4

Pulsatile, high dose sunitinib has no effect on tumor microvessel density (MVD) or on proliferation rate of cancer cells. a Representative photos of (immunohistochemical) staining of tumors established on the CAM from HT-29 tumor cells, treated as indicated. Upper panel: CD31 staining; lower panel: Ki-67 staining b Quantification of microvessel density (MVD) using CD31 staining. Data are expressed as number of CD31 positive cells per field, x10 magnification. Scale bars, 100 μm c Quantification of tumor cell proliferation using Ki-67 staining. Data are expressed as percentage of Ki-67 positive tumor cells to total tumor cells per field, 40x magnification. Scale bars, 50 μm.Error bars, SD