Fig. 2

Snail is a direct target of miR-137 and miR-34a in OC cells. a Relative miR-34a expression in OC cell lines (SKOV-3 and ES-2) and normal ovarian epithelial NOEC cells. b, c ES-2 cells were transfected with reporter constructs containing either wild-type (WT) Snail, or Snail 3′-UTR with mutation (MUT), along with miR-137 mimic (b), miR-34a mimic (c), or negative control mimic (Neg mimic), respectively. Relative luciferase activity was measured. d, e qPCR analysis of Snail expression in OC cells after overexpression (d) or knockdown (e) of miR-137 and miR-34a. f Western blotting analysis of Snail expression in OC cells after overexpression or knockdown of miR-137 and miR-34a. g, h qPCR analysis of indicated mRNAs in OC cells after transient overexpression or knockdown of miR-137 (g) and miR-34a (h). i Relative mRNA expression of Snail in OC tissues and matched normal tissues. j Analysis of Snail mRNA expression using microarray (Oncomine) on normal ovary versus OC tissue. ^** P < 0.01