Fig. 3

MiR-137 and miR-34a inhibit EMT, invasion and sphere-forming ability of OC cells. a, b Cellular morphology of OC cells after transient knockdown (a) or overexpression (b) of miR-137 and miR-34a. Scale bar = 200 μm (a) and 100 μm (b). c, d Cell invasion (c) and sphere formation (d) of OC cells after transient knockdown or overexpression of miR-137 and miR-34a. e, f qPCR analysis of indicated mRNAs in SKOV-3 (e) and ES-2 (f) cells after transient knockdown or overexpression of miR-137 and miR-34a. g Representative images of invaded SKOV-3 cells after transient transfection with miR-137 inhibitor or miR-34a inhibitor or negative control inhibitor (Neg inhibitor). h Representative images of spheres formed from ES-2 cells transfected with miR-137 or miR-34a mimic or Neg mimic. Scale bar =100 μm. ^** P < 0.01