Fig. 1

Antigen and Poly(I:C) administration increases the ratio of CD11c⁺ CD8⁺ T cells. a EG7 tumor was implanted to wild-type mice (C57BL/6 J), and OVA or OVA + Poly(I:C) was injected around tumor at day 9 after tumor implantation. Tumor volume was measured every 2 to 3 days. b–d 6 days after OVA administration, the proportions of OVA-specific CD8⁺ T cells and CD11c⁺ CD8⁺ T cells were evaluated on flow cytometer. In panel b, the gating strategy is shown. The proportions of each cell populations in spleen (c) and DLN (d) are calculated. Error bars show ± SEM; n = 4 to 5 per group. Student’s t-test was performed to analyze statistical significance. * p <0.05. The results are the representatives of three independent experiments