Fig. 6

Poly(I:C) therapy induces the expansion of CD11c⁺ CD8⁺ T cells in a WT1 tumor model. a Wild-type mice challenged with WT1-C1498 tumor cells were treated with PBS or Poly(I:C) around tumor at day 5 and 12 after implantation. Tumor volume was measured every 2 to 3 days. b At day 14, spleen and tumor were harvested and the proportions of WT1-specific CD8⁺ T cells, CD11c⁺ CD8⁺ T cells and CD62L⁻ CD8⁺ T cells were evaluated on flow cytometer. c The representative mice were selected from the PBS and Poly(I:C) group. Tumor growth and the proportions of CD11c⁺ CD8⁺ T cells in spleen and tumor are shown. In the left panel, diamond indicates PBS-treated control. triangle indicates Poly(I:C) low-responding mice. Circle indicates Poly(I:C) high-responding ones. Error bars show ± SEM; n = 3 to 4 per group. Student’s t-test was performed to analyze statistical significance. * p < 0.05. The results are one of the two independent experiments