Fig. 7

CD11c⁺ CD8⁺ T cells expand in response to Ag immunization in human PBMCs. Human PBMCs isolated from donor-1 were treated with CMV pp65 protein in the presence or absence of Poly(I:C). Negative control was a sample without Ag and Poly(I:C). Eight days after the treatment, the proportions of CMV pp65 tetramer⁺ CD8⁺ T cells and CD11c⁺ CD8⁺ T cells were evaluated on flow cytometer. a The gating strategy is shown. Samples were labeled with 7AAD, α-CD8 Ab, α-CD3 Ab or isotype of α-CD11c Ab without tetramer staining. b Upper panels show the percentage of pp65 tetramer⁺ cells in CD8⁺ T cells. Lower panels show the percentage of CD11c⁺ cells in CD8⁺ T cells. c The percentage of pp65 tetramer⁺ cells in CD11c negative- and CD11c positive-CD8⁺ T cells are shown