Fig. 3

CPS induces mutp53 protein degradation. a U373 and SKBR3 cells were treated with CPS (200 μM) and chloroquine (CHQ) (25 μM) for 24 h. Protein levels were measured with western blot using antibodies to LC3-II and p53. Anti-β-actin was used as protein loading control. In the right panel data are presented as mean ± S.D. *P = 0.001. b H1299 cells were transiently transfected with pcDNA3-p53R175H (0.1 μg), pcDNA3-R273H (0.1 μg) and control pcDNA3 vectors and the day after transfection treated with CPS (200 μM) for 24 h. Cell death measurements were assayed by Tunel assay. Equal amount of total cell extracts was analysed by western immunoblotting with anti-p53 and anti-p62 antibodies. Anti-β-actin was used as protein loading control. The percentage of p53 reduction was measured by densitometry and plotted in the right panel. *P = 0.001