Fig. 3

Knockdown of lncRNA XIST expression inhibits tumor growth and metastasis in vivo. a and b The infection efficiency of lentivirus in SGC7901 cells. Because this vector contains a GFP fragment, the cells emit green fluorescence when infected by the virus. The SGC7901 cells were observed under light and green fluorescence microscopy (a), real-time PCR analysis confirmed the interference efficiency. c Knockdown of lncRNA XIST expression significantly inhibited tumor growth of SGC7901 cells, the mean tumor volume was 313 mm³ and 858 mm³ for the SGC7901/sh-XIST and SGC7901/sh-NC groups, respectively (*P < 0.05). d, e and f Knockdown of lncRNA XIST expression significantly reduced the metastatic nodules in the liver, the mean metastases nodules were 1.7 and 7.3 for the SGC7901/sh-XIST and SGC7901/sh-NC groups, respectively (*P < 0.05)