Fig. 5

lncRNA XIST regulates EZH2 expression by acting as a molecular sponge. a The predicted miR-101 binding sites in EZH2 mRNA 3′-UTR as predicted by Targetscan algorithm. b Luciferase reporter assay for EZH2 mRNA 3′-UTR following miR-101 ectopic expression (*P < 0.05). c and d EZH2 mRNA and protein level in SGC7901 cells following ectopic expression of miR-101 and/or EZH2 expression vector lacking the 3′-UTR (*P < 0.05). e and f EZH2 mRNA and protein level in SGC7901 cells following knockdown of lncRNA XIST and/or inhibition of miR-101 (*P < 0.05)