Fig. 2

The loss of WNT5A in HB2 cells induces changes in EMT markers. a Representative Western blots showing changes in EMT markers upon the treatment of HB2 cells with WNT5A siRNAs. Two sequence-specific siRNAs targeted against WNT5A were used in the present experiment (as described in the Methods section). The EMT markers b E-cadherin, c vimentin and d β-catenin were quantified by calculating the integrated densitometric values and normalizing them to the tubulin levels. Statistical comparisons were made with Student’s t-test (b, c and d). All error bars represent the standard error of the mean (n = 5). *p < 0.05, **p = 0.01