Fig. 5

WNT5A significantly decreases the migration and invasion of breast cancer cells. a A wound-healing migration assay and b a Transwell invasion assay were performed with the breast cancer cell line MDA-MB468 in either the presence or absence of rWNT5A (0.4 μg/ml) for 72 h. Vehicle-treated MDA-MB468 cells were used as controls. Similarly, the relative migration and invasion (c and d) of the breast cancer cell line MDA-MB231 were quantified in the presence or absence of rWNT5A (0.4 μg/ml) for 72 h. Vehicle-treated MDA-MB231 cells were used as controls. Statistical comparisons were made with Student’s t-test (a, b, c, and d). All error bars represent the standard error of the mean (n = 4). *p < 0.05, ***p = 0.001