Fig. 6

WNT5A significantly inhibits CD44 and pAKT expression in breast cancer cells. a CD44 expression was analyzed in MDA-MB468-EV cells and MDA-MB468-5A cells by Western blotting. The CD44 levels in MDA-MB468-EV and MDA-MB468-5A cells were quantified by calculating integrated density values and normalizing them to the tubulin levels. b CD44 expression was analyzed in MDA-MB231-EV cells and MDA-MB231-5A cells by Western blotting. CD44 levels in MDA-MB231-EV and MDA-MB231-5A cells were quantified by calculating integrated density values and normalizing them to tubulin levels. pAKT activation was analyzed in MDA-MB468-EV cells and MDA-MB468-5A cells as in panel c and in MDA-MB231-EV cells and MDA-MB231-5A cells as in panel d. pAKT levels were quantified by calculating integrated density values and normalizing them to total AKT levels. Statistical comparisons were made with Student’s t-test (a, b, c and d). All error bars represent the standard error of the mean (n = 5). *p < 0.05, **p = 0.01, ***p = 0.001