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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Demystifying the mechanistic and functional aspects of p21 gene activation with double-stranded RNAs in human cancer cells

Fig. 2

Moment analysis of the kinetics of dsRNA subcellular distribution and RNAa activity. a Schematic representation of modified dsRNAs (dsP21-322 or dsControl) that were covalently linked to Cy3 at the 5’-end of either the sense (dsRNA-S-5’Cy3) or antisense (dsRNA-AS-5’Cy3) sequences. The sense strand of each duplex is shown in black, and the antisense strand is shown in red. b PC-3 cells were transfected with 50 nM of fluorescently labeled dsRNAs for 24 h or c 48 h to monitor the dsRNA subcellular distribution. d PC-3 and T-24 cells were transfected with 50 nM dsP21-322 for the indicated lengths of time to monitor target gene induction via RNAa. Expression levels of p21 were assessed using RT-qPCR. 18S RNA was also evaluated and served as a loading control. The results are expressed as the mean ± S.D. of three independent experiments. e p21 and 18S rRNA expression levels were assessed using RT-qPCR in PC-3 and T-24 cells after dsRNA or mock transfections. The p21 expression level was normalized to that of 18S rRNA and plotted as the fold change relative to the mock treatment. The results are expressed as the mean ± S.D. of three independent experiments

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