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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Demystifying the mechanistic and functional aspects of p21 gene activation with double-stranded RNAs in human cancer cells

Fig. 5

Enrichment of AGO1 and H3K4me3 at dsP21-322-targeted promoters or the p21 transcription start site (TSS). a Schematic representation of the locations of the PCR primers used for scanning ChIP analysis of 1.5 kb of the proximal p21 promoter. The locations are shown relative to the TSS. A ChIP assay was performed using an anti-AGO1 or anti-H3K4me3 antibody to pull down the p21 promoter upstream relative to the TSS (b and c), dsP21-322-targeted promoters d or p21 TSS-associated with AGO1 or H3K4me3 e in PC-3 and T-24 cells. The resulting DNA was amplified using qPCR and normalized to input levels. The input consists of nuclear extract prior to treatment with the antibody. IgG serves as the negative control antibody. The results are expressed as the mean ± S.D. calculated from triplicate independent transfection experiments with triplicate qPCR measurements for each

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