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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: eEF1Bγ binds the Che-1 and TP53 gene promoters and their transcripts

Fig. 5

eEF1Bγ depletion: effects on DNA damage response a HCT116 cells were transiently transfected with either siRNA-eEF1Bγ or siRNA-Control and 72 h later were treated with 1 μM doxorubicin (Dox) at various time points as indicated. Quantitative real time RT-PCR (qPCR) analysis was performed. The gene expression ratio of eEF1Bγ, Che-1 and p53, normalized as indicated, are shown as the mean ± SD from three independent experiments performed in triplicate. b Representative western blot of HCT116 cells (siRNA-Control and siRNA-eEF1Bγ) treated with 1 μM Dox at various time points as indicated. The antibodies that were used are indicated. Densitometric analysis represents the mean ± S.D. of four independent experiments (right panel)

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