Fig. 1

HSP70-2 gene and protein expression in clinical specimens and breast cancer cell. a Representative gel picture of RT-PCR analysis shows HSP70-2 gene expression in different histotypes (DCIS, IDC, ILC), stages (I-IV) and grades (1–3) of breast cancer specimens. b Representative images of IHC analysis of tissue sections show HSP70-2 protein expression (chocolate brown reactivity) in different histotypes (DCIS, IDC, ILC), stages (I-IV) and grades (1–3) of breast cancer specimens DCIS: Ductal Carcinoma in-situ, IDC: Infiltrating Ductal carcinoma and ILC: Infiltrating Lobular Carcinoma. Objective: ×20, Magnification: ×200. c RT-PCR analysis shows HSP70-2 gene expression in breast cancer cells: BT-474, MCF7, MDA-MB-231 and SK-BR-3. However, no HSP70-2 gene expression was observed in human normal mammary epithelial cells (HNMEC’s). Testis was used as positive control and β-actin as loading control. d Histogram shows the qPCR analysis depicting higher expression of HSP70-2 gene in breast cancer cells as compared to HNMECs. e Western blot analysis shows HSP70-2 protein expression in all breast cancer cells compared to no expression in HNMECs. f Flow cytometric analysis showed surface localization (displacement of fluorescence intensity on X-axis) of HSP70-2 protein in all breast cancer cells. Red peak shows unstained population, black peak shows cell population stained with control IgG antibody and green peak shows cell population probed with anti-HSP70-2 antibody. g IIF depicts co-localization (yellow-orange staining) of HSP70-2 in endoplasmic reticulum, mitochondria and Golgi bodies. However, no co-localization was observed in nuclear envelope. Nucleus is stained with DAPI. Objective: ×63, Magnification: ×630