Fig. 5

HSP70-2 shRNA treatment reduces tumor growth of human breast cancer xenograft. a Representative image shows reduced tumor size of the mice treated with shRNA4 (n = 8) compared to NC shRNA (n = 8). b Histogram shows significant difference in weight of tumor when treated with shRNA4 compared to NC shRNA. c Line graph shows significant difference in tumor volume when treated with shRNA4 compared to NC shRNA. d Western blot analysis depicts decreased expression of HSP70-2 and PCNA in shRNA4 treated tumor as compared to NC shRNA. e Representative images show IHC analysis of serial sections of shRNA4 and NC shRNA treated tumor for HSP70-2, PCNA and cell cycle molecules (CDK1, CDK2, CDK4, CDK6, Cyclin A2, Cyclin B1, Cyclin D1, Cyclin E, p21). H&E staining shows cytostructure of the tumor cells. Objective: ×20, Magnification: ×200. *P < 0.05, **P < 0.001. Data are represented as mean ± SEM