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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: TLE3 represses colorectal cancer proliferation by inhibiting MAPK and AKT signaling pathways

Fig. 2

Overexpression of TLE3 repressed the proliferation and tumorigenesis of human CRC cells. a Overexpression of TLE3 in SW480 and Ls174t cells. α-Tubulin was used as a loading control. b Overexpression of TLE3 repressed cell proliferation of SW480 and Ls174t cells by MTT assays. c Overexpression of TLE3 repressed cell proliferation of SW480 and Ls174t cells by colony formation assays. d Overexpression of TLE3 repressed anchorage-independent growth ability of SW480 and Ls174t cells as determined by soft agar assays. Colonies containing more than 50 cells were scored. e Tumorigenesis assay by subcutaneous injection of SW480/vector or SW480/TLE3 cells in nude mice (n = 6/group). Tumor volumes were measured on the indicated days. Data points are the mean tumor volumes ± SD. p < 0.05. f The sections of tumor were under H&E staining or subjected to IHC staining using an antibody against Ki-67. Error bars represent the mean ± SD of 3 independent experiments. * p < 0.05

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