Fig. 3

Knock-down of TLE3 promoted the proliferation and tumorigenesis of human CRC cells. a RNAi-silencing of TLE3 in shRNA-transduced stable HCT15 and SW620 cells. α-Tubulin was used as a loading control. b Knock-down of TLE3 promoted cell proliferation of HCT15 and SW620 cells by MTT assays. c Knock-down of TLE3 promoted cell proliferation of HCT15 and SW620 cells by colony formation assays. d Knock-down of TLE3 promoted anchorage independent growth ability of HCT15 and SW620 cells as determined by soft agar assays. Colonies containing more than 50 cells were scored. e Tumorigenesis assay by subcutaneous injection of SW620/Scr and SW620/sh-1 cells in nude mice (n = 6/group). Tumor volumes were measured on the indicated days. Data points are the mean tumor volumes ± SD. f The sections of tumor were under H&E staining or subjected to IHC staining using an antibody against Ki-67. Error bars represent the mean ± SD of 3 independent experiments. * p < 0.05