Fig. 3

Functional roles of TIMP1 in vitro. a QPCR showed that TIMP1 was knocked down in HCT116 and SW620 cells; cyclinD1 was decreased while p21 and p27 was upregulated in TIMP1-KD cells. b western blot confirmed that TIMP1, CyclinD1 was decreased while P21 and P27 was upregulated in TIMP1-KD cells. c TIMP1-KD inhibited CRC cell growth in vitro. d TIMP1-KD inhibited CRC cell colony formation in vitro. e Cell would healling ability was impaired in TIMP1-KD cells (200×). f Chamber invision ability was damaged in TIMP1-KD cells (200×) (scale bar = 20 μm)