Fig. 4

TIMP1 promotes CRC metastasis through EMT. a QPCR showed that E-cadherin was upregulated while SLUG and Fibronectin was downregulated in TIMP1-KD cells. b Western blot confirmed that E-cadherin was upregulated while SLUG and Fibronectin was downregulated in TIMP1-KD cells. c Immunofluorescence showed that E-cadherin was upregulated while Fibronectin was downregulated in TIMP1-KD cells d The relative expression of E-cadherin and Fibronectin in Scramble and TIMP1-KD cells (scale bar = 25 μm)