Fig. 6

DHQ3 and 17-DR induced the degradation of Hsp90 client proteins. a Hsp90 ATPase activity at different concentrations of GA, DHQ3 and 17-DR were tested by the malachite green-phosphate and ammonium molybdate method. At the given times, the reaction was stopped and the OD₆₅₀ value measured. b Cells were treated with analogs at different concentrations or different lengths of time, the Hsp90 client proteins were assessed by western blot. c MDA-MB-231 cells were pretreated with 10 μM MG132 for 4 h, with or without a subsequent 24 h incubation with 200 μM DHQ3 or 200 μM 17-DR. Whole-cell lysates were subjected to western blot analysis