Fig. 2

Internalization of MHCC-97H-derived exosomes in SMMC-7721-GFP cells. SMMC-7721-GFP cells in culture were incubated with MHCC-97H-derived exosomes labeled with CM-DIL (red). Cells were fixed with polyformaldehyde and mounted with ProLong Gold Antifade Reagent, as described in Materials and Methods. Low-magnification images of SMMC-7721-GFP cells incubated with exosomes (a, b, c). High-magnification images of SMMC-7721-GFP cells incubated with exosomes (d). MHCC-97H-derived exosomes were shown to be internalized in the cytoplasm of SMMC-7721-GFP cells