Fig. 1

Deregulated expression of the long non-coding RNA HOTTIP in gliomas and glioma cell lines and over-expression of HOTTIP inhibited glioma cell proliferation. a HOTTIP expression was assessed by real-time PCR using SYBR Green in human glioma tissues and normal tissues. The 2^−DDCt method was used to quantify the relative gene expression levels. b HOTTIP expression was assessed by real-time PCR using SYBR Green in four human glioma cell lines (U87-MG, U118-MG, A172 and U251) and astrocyte cells. The 2^−DDCt method was used to quantify the relative gene expression levels. c Stable over-expression of HOTTIP in U87-MG, and the cck-8 assay was performed to determine proliferation at the indicated time points. d Stable over-expression of HOTTIP in U118-MG, and the cck-8 assay was performed to determine proliferation at the indicated time points. The results represent data from at least three independent experiments expressed as the mean ± SD.*P <0.05