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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Over-expression of the long non-coding RNA HOTTIP inhibits glioma cell growth by BRE

Fig. 4

Over-expression of HOTTIP directly decreased BRE expression. a and b Over-expression of HOTTIP-mediated knockdown of BRE by real-time PCR using SYBR Green and Western blot analysis in the U87-MG and U118-MG glioma cell lines. U6 and β-actin were used as references. The results represent data from at least three independent experiments expressed as the mean ± SD. Significant differences among the groups were assessed by one-way ANOVA. *P < 0.05. c Cellular lysates from U87-MG to U118-MG cells were used for RNA immunoprecipitation (RIP) with the BRE antibody. Detection of BRE using IP-Western. d RIP was performed using the BRE antibody to immunoprecipitate HOTTIP and a primer to detect HOTTIP. e The sequence of BRE response element of wide type (wt) or mutated (mt) and HOTTIP. f Luciferase activity of the wild-type binding sites on BRE was significantly suppressed by HOTTIP compared with the lncRNA control. The results represent data from at least three independent experiments expressed as the mean ± SD. (**P < 0.01)

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