Fig. 4
miR-501-5p activates wnt/β-catenin signaling pathway. a TOP/FOP luciferase reporter assays in indicated cells. b Western blotting analysis of β-catenin in nuclear fraction of cells. The nuclear protein p84 was used as the nuclear protein marker. MEK1/2 was used as the cytoplasmic marker. c Real-time PCR analysis revealed that miR-501-5p regulates the expression levels of multiple wnt/β-catenin downstream genes in gastric cancer cells. The pseudocolors represent the intensity scale of expression in miR-501-5p vs. control cells, or antagomiR-501-5p vs. control cells generated by log2 transformation. d The stimulatory effect of miR-501-5p on TOP/FOP luciferase reporter activity was impaired by silencing β-catenin or expressing TCF4-dn. e Sphere formation assays indicated that silencing β-catenin or expressing TCF4-dn abrogated the promotive effects of miR-501-5p on self-renewal of gastric cancer cells. Error bars represent the mean ± s.d. of three independent experiments. *P < 0.05