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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: LCL161 increases paclitaxel-induced apoptosis by degrading cIAP1 and cIAP2 in NSCLC

Fig. 5

Paclitaxel increases TNFα expression, and LCL161 decreases cIAP1 and cIAP2 expression. a A549 and H460 cells were treated for 48 h with 0–10 μM paclitaxel. The protein levels of TNF-α and cleaved caspase-8 and cleaved caspase-3 were assessed by western blotting. b Cells were treated for 24 h with 0–10 μM paclitaxel. The level of TNFα in the cell culture media was quantified using the Human TNFα ELISA Kit. c A549 and H460 cells were treated for 48 h with 0–20 μM LCL161. The protein levels of cIAP1, cIAP2, XIAP and survivin were assessed by western blotting. β-actin was used as a loading control. The bar graphs represent the mean ± SD of different proteins/β-actin; *P < 0.05; **P < 0.01; ***P < 0.001

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