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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: LCL161 increases paclitaxel-induced apoptosis by degrading cIAP1 and cIAP2 in NSCLC

Fig. 7

LCL161 and paclitaxel cooperate to activate caspase-3 and caspase-8. A549 and H460 cells were treated for 24 h with 10 μM LCL161 and/or 10 μM paclitaxel. The changes in caspase-8 (a) and caspase-3 (b) activity were determined as described in the Materials and Methods section. Data are provided as mean ± SD, *P < 0.05; **P < 0.01; ***P < 0.001. c NSCLC cells were treated for 24 h with the combination of 10 μM LCL161 and 10 μM paclitaxel in the presence or absence of 20 μM caspase-8 inhibitor Z-IETD-FMK. Caspase-8 activity was detected using a caspase-8 activity assay kit. d Annexin V/PI staining was used to detect apoptosis, and the apoptotic rates were provided as mean ± SD; *P < 0.05; **P < 0.01; ***P < 0.001

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