Fig. 2

MiR-342-3p was a target of H19. a Expression level of miR-342-3p in pair samples of GBC and normal tissues was detected by qRT-PCR (n = 35, P < 0.05). The expression of miR-342-3p was normalized to U6. b Pearson’s correlation was used for correlation analysis between the expression of H19 mRNA and miR-342-3p mRNA (R = −0.403, P < 0.05). c Representation the H19 and miR-342-3p binding site by miRanda (http://www.microrna.org/). d Luciferase activity in HEKT293 cells cotransfected with miR-342-3p mimic and luciferase reporters containing control vector, pmir-Glo-H19-wt and pmir-Glo-H19-mut (P < 0.05). e Pull-down assay was conducted using Biotin-labeled H19 probe and detected the AGO2 expression by western-blotting assays. Antisense of the H19 probe was used as negative control. f Pull-down assay was conducted using Biotin-labeled H19 probe and miR-342-3p level was detected in the substrate of pull-down assay by qRT-PCR, antisense of the H19 probe was used as negative control. g H19 mRNA level was detected in the substrate of RIP assay by qRT-PCR. All data were represented as the mean ± S.D. from three independent experiments, **P < 0.05