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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Shock wave-induced ATP release from osteosarcoma U2OS cells promotes cellular uptake and cytotoxicity of methotrexate

Fig. 4

P2X7 was highly expressed on the cell surface and shock waves increased the extracellular concentration of ATP in U2OS cells. a P2X7 receptor expression in U2OS cells. Representative images of anti-P2X7 antibody separate experiments are shown. b Cells treated with BFA, GdCl3, and shock waves, and extracellular ATP concentrations were assessed in supernatants of the treated cells as indicated. Data are mean ± SD, n = 3, *p <0.05, Student, s t-test

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