Fig. 5

Alteration of cell membrane permeability by ATP/P2X7 contributed to the promotion of cell apoptosis of MTX by shock waves. a U2OS cells were treated with the indicated concentrations of ATP and cell viability was assessed by MTT assay. b U2OS cells were treated with MTX, shock waves and KN62 as indicated and apoptosis was assessed. Shown are representative histograms. c Quantitation of the apoptosis rates. Data are mean ± SD, n = 3, *p < 0.05,Student^,s t-test