Fig. 2

Silencing RARγ impaires HCC migration and invasion in vitro and metastasis in vivo. a–d Migration (a) and invasion (c) assays were performed in wild-type MHCC-97H cells (shRNA/Control) and in the MHCC-97H cells with stable knock down of RARγ (shRNA/ RARγ), and the relative number of migratory (b) and invasive (d) cells were calculated with Wright-Giemsa staining. e Knockdown of RARγ inhibits HCC metastasis. Representative lung tissue sections from each group were shown by hematoxylin and eosin staining (magnification: × 40). Black arrows indicate lung tissues with matastatic nodules. f The number of lung metastatic foci in each group (n = 6 per group) was counted under the microscope. Statistical significance was determined by a two-tailed, unpaired Student's t-test. **p < 0.01