Fig. 7

Functional analysis of spheroid-derived endothelial cells (spheroid-EC). a Morphological analysis of spheroid cells, spheroid-EC differentiated at 10 ng/ml VEGF, and HUVEC. b Flow cytometry analysis of CD31 and CD34 expression in spheroid cells, spheroid-EC, and HUVEC. c1,c2 The comparative expression of CD31 and CD34 in spheroid cells, spheroid-EC, and HUVEC. d Assessment of network formation by spheroid cells, spheroid-EC, and HUVEC on Matrigel. e Fluorescence imaging of acetylated low-density lipoprotein (red) and spheroid cells, spheroid-EC, and HUVEC metabolizing ac-LDL