Fig. 6

Immunophenotyping of monocyte derived dendritic cells pulsed with either tumor lysate or tumor lysate containing nanoparticles, a DCs treated with tumor antigen pulsed nanoparticles fabricated using three different amounts (concentrations) of PVA, Our results showed that NP3 pulsed DCs expressed upper levels of, HLA-DR and lower levels of CD14 and CD86 markers significantly (P ≤ 0.05), while expression of CD83 and CD80 markers were significantly up and down-regulated respectively (P ≤ 0.05). b Comparison of DCs pulsed with nanoparticle containing tumor antigen with those pulsed with either tumor lysate or NPs alone, the data of this study showed that the tumor lysate loaded NPs triggers a more efficient maturation of monocyte-derived DCs compared to either tumor lysate or NPs alone (P ≤ 0.05) Depicted data are averaged from three independent samples obtained from breast cancer patients (n = 3) (*: indicates the statistical significance)