Fig. 1

The protein and mRNA expression of TRIM62 determined by western blot, qRT-PCR and IHC assays. The expression level of TRIM62 was normalized to β-actin. The error bars represent standard deviation values calculated from three parallel experiments. NCT, normal cervical tissue; CC, cervical cancer; SCC, squamous cell carcinoma; AC, adenocarcinoma; NS, no statistical significance; **, P < 0.01; ***, P < 0.001. a Analysis of the mRNA expression level of TRIM62 in eight human CC cell lines by qRT-PCR. b Western blot analyzed the protein expression level of TRIM62 in eight human CC cell lines (left). Quantitative analysis of TRIM62 protein expression in eight human CC cell lines was displayed (right). c Utilizing qRT-PCR assays, TRIM62 mRNA expression were detected in 20 NCT and 40 early-stage CC tissues. There were 28 SCC and 12 AC tissues. d Western blot assay of TRIM62 protein expression were detected in 8 NCT and 8 early-stage CC tissues, including 4 SCC and 4 AC tissues (left). Quantitative analysis of TRIM62 protein expression in NCT and CC tissues was shown (right). e IHC assays were performed for investigating TRIM62 protein expression in six pairs of matched cervical cancer (T) samples and adjacent nontumor cervical tissue (ANT) samples. The TRIM62 expression located in the cytoplast was strong in NCT of ANT samples, weak or negative in T samples. Original magnifications: ×200